There are many youtube videos on how to run the machine 

Manual settings for mach 3

The steps per unit are calculated as follows

 motors 200 steps per rev

 X motor drive 10  microsteps X motor turns per inch

200X10X20=40000 steps per inch

Mach 3 db 25 Pin set up

X axis step pin 2 direction pin 3

Y axis step pin 4 direction pin 5

Z axis step pin 6 direction pin 7

A axis step pin 8 direction pin 9

Motor tuning setting

acceleration of 4.5-5  velocity of 60